Our Research & Patents

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Microorganism having predacious ability for soil nematoda parasitic on plant from soil, and method for incubating the same

PURPOSE: Provided are plant-parastic soil nematophagous microorganism, Arthrobotrys sp. microorganism(KCTC 8992P), devouring soil nematodes which damage the crops and being applied to insecticides. And its culture method is also provided. 

CONSTITUTION: The plant-parastic soil nematophagous microorganism, Arthrobotrys sp. microorganism(KCTC 8992P) is obtained by the steps of: sieving soil with a 1.0 mm sieve; suspending sieved soil with sterilized distilled water; centrifuging suspended solution and separating supernatant and precipitates; inoculating the precipitates on a corn meal 2 agar medium and culturing at 25 deg.C for 24 hours; adding 200-300 plant parastic nematophagous microorganisms thereto then culturing them at 25 deg.C for 7 days with aseptic culture technique to form spores of the plant parastic soil nematophagous microorganism; transplanting the spores to another corn meal 2 agar medium followed by culturing them; and re-culturing the culture with pure-culture technique, wherein a medium therefor is prepared by mixing 1.0-3.0 wt.% of peptone; 1.0-3.0 wt.% of beef-extract; 0.5-1.5 wt.% of yeast extract; 4.0-7.0 wt.% of glucose; 0.02-0.05 wt.% of KH2PO4; 0.003-0.008 wt.% of MgSO4; 0.003-0.008 wt.5 of KCl; and a balance of distilled water, and its pH is adjusted to pH 5-7 by using NH4OH.

Probiotics composition for mammalian feed supplements

The present invention relates to a probiotic composition capable of exhibiting the red pigment effect of astaxanthin in mammals by the interaction of papia rhodoma and other beneficial bacteria. In the present invention, by feeding the papia Rhododima producing natural astaxanthin to livestock together with Lactobacillus crispatus Gyesan (KCTC-10326BP), which is known to be excellent in improving the intestinal microbial environment of livestock, It is intended that the efficacy of astaxanthin is shown in mammalian livestock The present invention provides a composition in the form of a complex probiotic that supplements Papia rhododoma with Lactobacillus crispatus calculation, according to the present invention, the red color of astaxanthin Coloring can improve the color of meat and improve the carcass grade and preservation.

Probiotics composition for feed supplements

Provided is a probiotic composition for a livestock feed supplement, which allows the effects of astaxanthin to be realized in mammals, and thus improves a color degree, quality and preservability of meat. The probiotic composition for a livestock feed supplement comprises 1.0X10^6 cfu/g to 1.0X10^9 cfu/g of Phaffia rhodozyma and 1.0X10^6 cfu/g to 1.0X10^9 cfu/g of Lactobacillus crispatus Gyesan(KCTC-10326BP). The probiotic composition optionally further comprises at least one of 1.0X10^6 cfu/g to 1.0X10^9 cfu/g of Lactobacillus plantarum, 1.0X10^6 cfu/g to 1.0X10^9 cfu/g of Saccharomyces cerevisiae and 1.0X10^6 cfu/g to 1.0X10^9 cfu/g of Enterococcus faecium.

Novel bacteriocin-producing lactic acid bacteria and mixed microbial composition using it for livestocks

The present invention relates to a lactic acid strain producing bacteriocin and a complex probiotic composition for livestock containing the same. In the present invention, pig and of the digestive system, and the three strains that are each derived from a traditional fermented foods, kimchi production of antibacterial substances, Lactobacillus AP 116 (Lactobacillus sp. AP 116 , KACC 91396P), Enterococcus paesyum CB 712 (Enterococcus faecium CB 712, KACC 91395P), Pediococcus pentosassius K23-2 ( Pediococcus pentosaceus K23-2, KACC 91397P) are isolated and identified. Moreover, in this invention, the complex probiotic composition using these is provided.

Lactobacillus Producing Bacteriocin and Probiotic Composition Containing the Same

The present invention relates to lactic acid bacteria producing an antimicrobial substance and a probiotic composition containing the same. In the present invention, four strains derived from the digestive tract of ducks and produce antibacterial substances, Lactobacillus salivarius JWS 58 (KACC91357P); Lactobacillus plantarum JWS 1354, KACC91358P); Enterococcus faecium JWS 833, KACC91359P and Pediococcus pentosaceus JWS 939 ( Pediococcus pentosaceus JWS 939, KACC91360P) are isolated and identified. Moreover, in this invention, the complex probiotic composition using these is provided.

Novel bacteriocin-producing lactic acid bacteria and mixed microbial composition using it for broiler chickens

PURPOSE: A microbial composition for chicken containing lactobacillus producing antibacterial ingredient is provided to ensure high suppression ability to fowl pathogenic composition. 

CONSTITUTION: A microbial composition contains Enterococcus faecium SH 528(KACC 91383P), Enterococcus faecium SH 632(KACC 91382P), and Pediococcus pentosaceus SH 740(KACC 91381P). The microbial composition is added to chicken feed or fed to chicken for replacing antibiotics.

Method of preparing jujube probiotics using solid-state fermentation for livestocks and use of it

PURPOSE: A method for preparing probiotics composition of solid fermentation for livestocks using jujube is provided to quickly and uniformly produce a large amount of products. 

CONSTITUTION: A method for preparing probiotics agent using jujube comprises: a first step of culturing Lactobaicllus, Bacillus subtilis, yeast(Saccharomyces cerevisiae), and Phaffia rhodozyma in a liquid medium containing 5-20 weight% of jujube extract at 25-40°C for 24-48 hours; a second step of adding 5-20 weight% of jujube extract to a basic medium containing rice bran, wheat bran, and molasse; and a third step of inoculating spawn of the liquid culture of first step and fermenting at 25-35°C for 2-4 days. The jujube extract is prepared by hot-water extraction of dried jujube and fresh jujube at 100-150°C for 3-8 hours.

A Method of Preparing the Insecticidal Extract of Sophorae Radix for Moth, the Agents comprising it and the Method of Controling Moth

The present invention relates to a ginseng root extract showing a moth moisturizing activity and a moth using the extract, in the present invention, after extracting the ginseng root in a dark state at room temperature for 40 to 60 hours with ethanol to obtain the extract by filtration, it is a reduced pressure concentrator After concentrating at 30 ~ 43 ℃ to provide a method for producing a ginseng root extract to be lyophilized and a moth-proof composition using the obtained ginseng root extract and a moth control method using the same. Since the ginseng root extract according to the present invention alone exhibits excellent moth control activity of more than 80% to 90%, it can be used as an effective mothicide in place of chemical pesticides that have been used.

A Method of Preparing the Aphicidal Extract of Sophorae Radix, the Agents Comprising it and the Method of Controling Aphids

The present invention relates to a ginseng root extract exhibiting aphid control activity and aphid control using this extract, in the present invention, the extract is extracted by ethanol 40 ~ 60 hours ethanol in the dark at room temperature and filtered to obtain an extract, Provided is a method for producing a ginseng root extract, which is concentrated at 30 to 43 ° C. with a concentrator and freeze-dried, and an aphid control composition using the obtained ginseng root extract and aphid control method using the same. Since the ginseng root extract according to the present invention alone exhibits excellent aphid control activity of more than 80%, it can be used as an effective aphid control agent in place of the chemical pesticide that has been used.

Composition and method of controlling fungi for crops and its preparation method

The present invention relates to a fungal plant disease control composition using rapeseed oil, a preparation method thereof and a control method using the same. In the present invention, 100 parts by weight of rapeseed oil; 6 to 17 parts by weight of polyoxyethylene oyl amine; 6 to 17 parts by volume of ethoxylated castyl oil; And a control agent stock solution composition for controlling fungal plant diseases containing 6 to 17 parts by volume of oleyl alcohol, and a control agent diluted in 100 to 2,000 times water by volume ratio, a preparation method and a control method using the same Is provided. The fungal plant disease control agent of the present invention has very good control value against fungal plant diseases such as powdery mildew, no harm to crops, and is low in toxicity and safe for human beings and the environment. The control agent of the present invention can be used to replace the existing chemicals for the prevention and treatment of fungal plant diseases of crops such as powdery mildew, it can be applied to environmentally friendly farming.

Composition for Controling Lycorma delicatula, the Methods of Preparing it and the Method of Controling Lycorma delicatula Using it

PURPOSE: A Lycorma delicatula repellent composition containing natural extract from plants is provided to replace agrochemical. 

CONSTITUTION: A Lycorma delicatula repellent composition contains 100 weight parts of Melia azedarach alcohol extract, 200-600 weight parts of Sophorae japonica alcohol extract, and 200-600 weight parts of Chenopodium alcohol extract. The alcohol extract is prepared at room temperature using 90-99% ethanol for 40-60 hours. The repellent composition further contains 5-15% ethanol solution and surfactant.